Detection of Wolbachia endobacteria in Culex quinquefasciatus by Gimenez staining and confirmation by PCR.
Identifieur interne : 004387 ( Main/Exploration ); précédent : 004386; suivant : 004388Detection of Wolbachia endobacteria in Culex quinquefasciatus by Gimenez staining and confirmation by PCR.
Auteurs : M. Muniaraj [Inde] ; R. Paramasivan ; I P Sunish ; N. Arunachalam ; T. Mariappan ; S Victor Jerald Leo ; K J DhananjeyanSource :
- Journal of vector borne diseases [ 0972-9062 ] ; 2012.
Descripteurs français
- KwdFr :
- ADN bactérien (analyse), ADN bactérien (génétique), Animaux, Coloration et marquage (), Culex (), Culex (microbiologie), Femelle, Filariose lymphatique (transmission), Ovaire (microbiologie), Réaction de polymérisation en chaîne (), Vecteurs insectes (microbiologie), Wolbachia (génétique), Wolbachia (isolement et purification).
- MESH :
- analyse : ADN bactérien.
- génétique : ADN bactérien, Wolbachia.
- isolement et purification : Wolbachia.
- microbiologie : Culex, Ovaire, Vecteurs insectes.
- Animaux, Coloration et marquage, Culex, Femelle, Filariose lymphatique, Réaction de polymérisation en chaîne.
English descriptors
- KwdEn :
- Animals, Culex (classification), Culex (microbiology), DNA, Bacterial (analysis), DNA, Bacterial (genetics), Elephantiasis, Filarial (transmission), Female, Insect Vectors (microbiology), Ovary (microbiology), Polymerase Chain Reaction (methods), Staining and Labeling (methods), Wolbachia (genetics), Wolbachia (isolation & purification).
- MESH :
- chemical , analysis : DNA, Bacterial.
- classification : Culex.
- chemical , genetics : DNA, Bacterial, Wolbachia.
- isolation & purification : Wolbachia.
- methods : Polymerase Chain Reaction, Staining and Labeling.
- microbiology : Culex, Insect Vectors, Ovary.
- transmission : Elephantiasis, Filarial.
- Animals, Female.
Abstract
Wolbachia are common intracellular bacteria that are found in arthropods and nematodes. These endosymbionts are transmitted vertically through host eggs and alter host biology in diverse ways, including the induction of reproductive manipulations, such as feminization, parthenogenesis, male killing and sperm-egg incompatibility. Since they can also move horizontally across species boundaries, Wolbachia is gaining importance in recent days as it could be used as a biological control agent to control vector mosquitoes or for paratransgenic approaches. However, the study of Wolbachia requires sophisticated techniques such as PCR and cell culture facilities which cannot be affordable for many laboratories where the diseases transmitted by arthropod vectors are common. Hence, it would be beneficial to develop a simple method to detect the presence of Wolbachia in arthropods.
PubMed: 23428527
Affiliations:
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Paramasivan</name></author><author><name sortKey="Sunish, I P" sort="Sunish, I P" uniqKey="Sunish I" first="I P" last="Sunish">I P Sunish</name></author><author><name sortKey="Arunachalam, N" sort="Arunachalam, N" uniqKey="Arunachalam N" first="N" last="Arunachalam">N. Arunachalam</name></author><author><name sortKey="Mariappan, T" sort="Mariappan, T" uniqKey="Mariappan T" first="T" last="Mariappan">T. 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Muniaraj</name><affiliation wicri:level="1"><nlm:affiliation>Centre for Research in Medical Entomology (ICMR), Madurai, Tamil Nadu, India. mmuniaraj@gmail.com</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Centre for Research in Medical Entomology (ICMR), Madurai, Tamil Nadu</wicri:regionArea><wicri:noRegion>Tamil Nadu</wicri:noRegion></affiliation></author><author><name sortKey="Paramasivan, R" sort="Paramasivan, R" uniqKey="Paramasivan R" first="R" last="Paramasivan">R. Paramasivan</name></author><author><name sortKey="Sunish, I P" sort="Sunish, I P" uniqKey="Sunish I" first="I P" last="Sunish">I P Sunish</name></author><author><name sortKey="Arunachalam, N" sort="Arunachalam, N" uniqKey="Arunachalam N" first="N" last="Arunachalam">N. Arunachalam</name></author><author><name sortKey="Mariappan, T" sort="Mariappan, T" uniqKey="Mariappan T" first="T" last="Mariappan">T. Mariappan</name></author><author><name sortKey="Jerald Leo, S Victor" sort="Jerald Leo, S Victor" uniqKey="Jerald Leo S" first="S Victor" last="Jerald Leo">S Victor Jerald Leo</name></author><author><name sortKey="Dhananjeyan, K J" sort="Dhananjeyan, K J" uniqKey="Dhananjeyan K" first="K J" last="Dhananjeyan">K J Dhananjeyan</name></author></analytic><series><title level="j">Journal of vector borne diseases</title><idno type="ISSN">0972-9062</idno><imprint><date when="2012" type="published">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Culex (classification)</term><term>Culex (microbiology)</term><term>DNA, Bacterial (analysis)</term><term>DNA, Bacterial (genetics)</term><term>Elephantiasis, Filarial (transmission)</term><term>Female</term><term>Insect Vectors (microbiology)</term><term>Ovary (microbiology)</term><term>Polymerase Chain Reaction (methods)</term><term>Staining and Labeling (methods)</term><term>Wolbachia (genetics)</term><term>Wolbachia (isolation & purification)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN bactérien (analyse)</term><term>ADN bactérien (génétique)</term><term>Animaux</term><term>Coloration et marquage ()</term><term>Culex ()</term><term>Culex (microbiologie)</term><term>Femelle</term><term>Filariose lymphatique (transmission)</term><term>Ovaire (microbiologie)</term><term>Réaction de polymérisation en chaîne ()</term><term>Vecteurs insectes (microbiologie)</term><term>Wolbachia (génétique)</term><term>Wolbachia (isolement et purification)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>DNA, Bacterial</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>ADN bactérien</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Culex</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Bacterial</term><term>Wolbachia</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN bactérien</term><term>Wolbachia</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Wolbachia</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Wolbachia</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Polymerase Chain Reaction</term><term>Staining and Labeling</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Culex</term><term>Ovaire</term><term>Vecteurs insectes</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Culex</term><term>Insect Vectors</term><term>Ovary</term></keywords><keywords scheme="MESH" qualifier="transmission" xml:lang="en"><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Female</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Coloration et marquage</term><term>Culex</term><term>Femelle</term><term>Filariose lymphatique</term><term>Réaction de polymérisation en chaîne</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Wolbachia are common intracellular bacteria that are found in arthropods and nematodes. These endosymbionts are transmitted vertically through host eggs and alter host biology in diverse ways, including the induction of reproductive manipulations, such as feminization, parthenogenesis, male killing and sperm-egg incompatibility. Since they can also move horizontally across species boundaries, Wolbachia is gaining importance in recent days as it could be used as a biological control agent to control vector mosquitoes or for paratransgenic approaches. However, the study of Wolbachia requires sophisticated techniques such as PCR and cell culture facilities which cannot be affordable for many laboratories where the diseases transmitted by arthropod vectors are common. Hence, it would be beneficial to develop a simple method to detect the presence of Wolbachia in arthropods.</div></front></TEI><affiliations><list><country><li>Inde</li></country></list><tree><noCountry><name sortKey="Arunachalam, N" sort="Arunachalam, N" uniqKey="Arunachalam N" first="N" last="Arunachalam">N. Arunachalam</name><name sortKey="Dhananjeyan, K J" sort="Dhananjeyan, K J" uniqKey="Dhananjeyan K" first="K J" last="Dhananjeyan">K J Dhananjeyan</name><name sortKey="Jerald Leo, S Victor" sort="Jerald Leo, S Victor" uniqKey="Jerald Leo S" first="S Victor" last="Jerald Leo">S Victor Jerald Leo</name><name sortKey="Mariappan, T" sort="Mariappan, T" uniqKey="Mariappan T" first="T" last="Mariappan">T. Mariappan</name><name sortKey="Paramasivan, R" sort="Paramasivan, R" uniqKey="Paramasivan R" first="R" last="Paramasivan">R. Paramasivan</name><name sortKey="Sunish, I P" sort="Sunish, I P" uniqKey="Sunish I" first="I P" last="Sunish">I P Sunish</name></noCountry><country name="Inde"><noRegion><name sortKey="Muniaraj, M" sort="Muniaraj, M" uniqKey="Muniaraj M" first="M" last="Muniaraj">M. Muniaraj</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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